Lab Technician I
Company: Unicon Pharma
Posted on: May 6, 2021
Job Description: This job function will support large scale cell
culture work and reagent generation in Structural Biology and
Biophysics. This position will be physically intensive requiring
the ability to lift and move large, heavy flasks. Duties may
include, but are not limited to: Prepare large scale cell reagents
using mammalian cells, E.coli and baculovirus with insect cells.
Propagate and maintain various continuous cell lines. Verify the
protein expression using various protocols including flow
cytometry, Western Blots, and small scale purification.
Experimental data will be recorded in a provided electronic
notebook according to the guidelines. Responsible for ordering
supplies as needed to deliver cell and protein reagents. As time
permits, support lab with generation of various solutions and some
molecular biology including mutagenesis and large scale plasmid
production. Coordination with various groups on delivery time-line
of cell pastes. Job Requirements: BS in biology or related field
with 0-2 years of relevant experience in mammalian and microbial
culture techniques including cell expansion and cryopreservation,
protein expression optimization and scale-up, protein expression
analysis by SDS-PAGE and Western Blot Methods. Excellent aseptic
technique is required. Experience using standard laboratory
equipment including incubators, microscopes, pipettes, and
biosafety cabinets. Ability to effectively supply a wide array of
cell reagents on a regular basis to support weekly requests is
critical. Ability to quickly identify and remedy potential issues
with cell culture. Ability to keep accurate records using
electronic notebooks and create common laboratory reagents
including buffers and cell culture media. Must be able to work on
multiple projects at one time at a time and have a positive
customer service focus. Computer skills required: Microsoft Word,
Excel, and Outlook. Ability to work in a high-throughput
environment, stay organized, and to adhere to specific timelines.
Team player with attention to detail, and excellent written and
verbal communication. Must be self-motivated, very reliable and
have a strong attention to detail.
Keywords: Unicon Pharma, New England , Lab Technician I, Professions , Groton, Northeast
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